1FYR

DIMER FORMATION THROUGH DOMAIN SWAPPING IN THE CRYSTAL STRUCTURE OF THE GRB2-SH2 AC-PYVNV COMPLEX

1FYR の概要

• エントリーDOI: 10.2210/pdb1fyr/pdb
• 分子名称: GROWTH FACTOR RECEPTOR-BOUND PROTEIN 2, HEPATOCYTE GROWTH FACTOR RECEPTOR PEPTIDE (3 entities in total)
• 機能のキーワード: grb2, sh2 domain, phosphopeptide, met, domain swapping, dimerization, hormone-growth factor complex, hormone/growth factor
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Golgi apparatus (By similarity): P29354; Membrane; Single-pass type I membrane protein: P08581
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 55522.44

構造登録者

Schiering, N.,Casale, E.,Caccia, P.,Giordano, P.,Battistini, C. (登録日: 2000-10-03, 公開日: 2000-12-06, 最終更新日: 2024-10-30)

主引用文献

Schiering, N.,Casale, E.,Caccia, P.,Giordano, P.,Battistini, C.
Dimer formation through domain swapping in the crystal structure of the Grb2-SH2-Ac-pYVNV complex.
Biochemistry, 39:13376-13382, 2000

PubMed Abstract: Src homology 2 (SH2) domains are key modules in intracellular signal transduction. They link activated cell surface receptors to downstream targets by binding to phosphotyrosine-containing sequence motifs. The crystal structure of a Grb2-SH2 domain-phosphopeptide complex was determined at 2.4 A resolution. The asymmetric unit contains four polypeptide chains. There is an unexpected domain swap so that individual chains do not adopt a closed SH2 fold. Instead, reorganization of the EF loop leads to an open, nonglobular fold, which associates with an equivalent partner to generate an intertwined dimer. As in previously reported crystal structures of canonical Grb2-SH2 domain-peptide complexes, each of the four hybrid SH2 domains in the two domain-swapped dimers binds the phosphopeptide in a type I beta-turn conformation. This report is the first to describe domain swapping for an SH2 domain. While in vivo evidence of dimerization of Grb2 exists, our SH2 dimer is metastable and a physiological role of this new form of dimer formation remains to be demonstrated.

PubMed: 11063574
DOI: 10.1021/bi0012336

実験手法

X-RAY DIFFRACTION (2.4 Å)