1FXA

CRYSTALLIZATION AND STRUCTURE DETERMINATION TO 2.5-ANGSTROMS RESOLUTION OF THE OXIDIZED [2FE-2S] FERREDOXIN ISOLATED FROM ANABAENA 7120

1FXA の概要

• エントリーDOI: 10.2210/pdb1fxa/pdb
• 分子名称: [2FE-2S] FERREDOXIN, FE2/S2 (INORGANIC) CLUSTER (3 entities in total)
• 機能のキーワード: electron transport
• 由来する生物種: Nostoc sp.
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 21762.89

構造登録者

Rypniewski, W.R.,Breiter, D.R.,Benning, M.M.,Wesenberg, G.,Oh, B.-H.,Markley, J.L.,Rayment, I.,Holden, H.M. (登録日: 1991-01-09, 公開日: 1992-07-15, 最終更新日: 2024-02-07)

主引用文献

Rypniewski, W.R.,Breiter, D.R.,Benning, M.M.,Wesenberg, G.,Oh, B.H.,Markley, J.L.,Rayment, I.,Holden, H.M.
Crystallization and structure determination to 2.5-A resolution of the oxidized [2Fe-2S] ferredoxin isolated from Anabaena 7120.
Biochemistry, 30:4126-4131, 1991

PubMed Abstract: The molecular structure of the oxidized form of the [2Fe-2S] ferredoxin isolated from the cyanobacterium Anabaena species strain PCC 7120 has been determined by X-ray diffraction analysis to a nominal resolution of 2.5 A and refined to a crystallographic R factor of 18.7%. Crystals used in this investigation belong to the space group P2(1)2(1)2(1) with unit cell dimensions of a = 37.42 A, b = 38.12 A, and c = 147.12 A and two molecules in the asymmetric unit. The three-dimensional structure of this ferredoxin was solved by a method that combined X-ray data from one isomorphous heavy-atom derivative with noncrystallographic symmetry averaging and solvent flattening. As in other plant-type [2Fe-2S] ferredoxins, the iron-sulfur cluster is located toward the outer edge of the molecule, and the irons are tetrahedrally coordinated by both inorganic sulfurs and sulfurs provided by protein cysteine residues. The main secondary structural elements include four strands of beta-pleated sheet and three alpha-helical regions.

PubMed: 1902376
DOI: 10.1021/bi00231a003

実験手法

X-RAY DIFFRACTION (2.5 Å)