1FWQ

SOLUTION STRUCTURE OF HUMAN MSS4, A GUANINE NUCLEOTIDE EXCHANGE FACTOR FOR RAB PROTEINS

1FWQ の概要

• エントリーDOI: 10.2210/pdb1fwq/pdb
• 分子名称: GUANINE NUCLEOTIDE EXCHANGE FACTOR, ZINC ION (2 entities in total)
• 機能のキーワード: zinc-binding, beta structure, metal binding protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13908.15

構造登録者

Yu, H.,Schreiber, S.L. (登録日: 2000-09-24, 公開日: 2000-10-04, 最終更新日: 2024-05-22)

主引用文献

Yu, H.,Schreiber, S.L.
Structure of guanine-nucleotide-exchange factor human Mss4 and identification of its Rab-interacting surface.
Nature, 376:788-791, 1995

PubMed Abstract: Guanine-nucleotide-exchange factors (GEFs) promote the exchange of GDP for GTP in Ras GTPases, and thereby positively regulate their functions. Members of the Sec4/Ypt1/Rab branch of the Ras superfamily are essential for vesicular transport. A GEF for a subset of Rab proteins, termed mammalian suppressor of Sec4 (Mss4), has been identified. Here we use multidimensional NMR to determine the structure of human Mss4 (hMss4), which is the first tertiary structure established for a protein with GEF activity. Mss4 contains a central beta-sheet sandwiched between two small sheets. It also binds a Zn2+ ion through Cys 23, Cys 26, Cys 94 and Cys 97. The Rab-binding surface of hMss4 has subsequently been delineated using chemical-shift perturbation experiments and site-directed mutagenesis. The active site of hMss4 involves the Zn(2+)-binding region and a neighbouring loop.

PubMed: 7651540
DOI: 10.1038/376788a0

実験手法

SOLUTION NMR