1FVI

CRYSTAL STRUCTURE OF CHLORELLA VIRUS DNA LIGASE-ADENYLATE

1FVI の概要

• エントリーDOI: 10.2210/pdb1fvi/pdb
• 分子名称: CHLORELLA VIRUS DNA LIGASE-ADENYLATE, SULFATE ION, ADENOSINE MONOPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: adenylated dna ligase, ligase
• 由来する生物種: Chlorella virus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34567.48

構造登録者

Odell, M.,Sriskanda, V.,Shuman, S.,Nikolov, D.B. (登録日: 2000-09-20, 公開日: 2000-11-22, 最終更新日: 2024-11-20)

主引用文献

Odell, M.,Sriskanda, V.,Shuman, S.,Nikolov, D.B.
Crystal structure of eukaryotic DNA ligase-adenylate illuminates the mechanism of nick sensing and strand joining.
Mol.Cell, 6:1183-1193, 2000

PubMed Abstract: Chlorella virus DNA ligase is the smallest eukaryotic ATP-dependent ligase known; it has an intrinsic nick-sensing function and suffices for yeast cell growth. Here, we report the 2.0 A crystal structure of the covalent ligase-AMP reaction intermediate. The conformation of the adenosine nucleoside and contacts between the enzyme and the ribose sugar have undergone a significant change compared to complexes of T7 ligase with ATP or mRNA capping enzyme with GTP. The conformational switch allows the 3' OH of AMP to coordinate directly the 5' PO(4) of the nick. The structure explains why nick sensing is restricted to adenylated ligase and why the 5' phosphate is required for DNA binding. We identify a metal binding site on ligase-adenylate and propose a mechanism of nick recognition and catalysis supported by mutational data.

PubMed: 11106756
DOI: 10.1016/S1097-2765(00)00115-5

実験手法

X-RAY DIFFRACTION (2 Å)