1FV3

THE HC FRAGMENT OF TETANUS TOXIN COMPLEXED WITH AN ANALOGUE OF ITS GANGLIOSIDE RECEPTOR GT1B

1FV3 の概要

• エントリーDOI: 10.2210/pdb1fv3/pdb
• 関連するPDBエントリー: 1a8d 1af9 1d0h 1dfq 1diw 1dll 1fv2
• 分子名称: TETANUS TOXIN HEAVY CHAIN, N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-galactopyranose-(1-4)-[N-acetyl-alpha-neuraminic acid-(2-8)-N-acetyl-beta-neuraminic acid-(2-3)]beta-D-galactopyranose-(1-4)-beta-D-glucopyranose, ETHYL-TRIMETHYL-SILANE, ... (5 entities in total)
• 機能のキーワード: toxin, carbohydrate, ganglioside, multi-valent binding, receptor
• 由来する生物種: Clostridium tetani
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 111457.81

構造登録者

Fotinou, C.,Emsley, P.,Black, I.,Ando, H.,Ishida, H.,Kiso, M.,Sinha, K.A.,Fairweather, N.F.,Isaacs, N.W. (登録日: 2000-09-18, 公開日: 2001-09-05, 最終更新日: 2024-11-20)

主引用文献

Fotinou, C.,Emsley, P.,Black, I.,Ando, H.,Ishida, H.,Kiso, M.,Sinha, K.A.,Fairweather, N.F.,Isaacs, N.W.
The crystal structure of tetanus toxin Hc fragment complexed with a synthetic GT1b analogue suggests cross-linking between ganglioside receptors and the toxin.
J.Biol.Chem., 276:32274-32281, 2001

PubMed Abstract: Tetanus toxin, a member of the family of Clostridial neurotoxins, is one of the most potent toxins known. The crystal structure of the complex of the COOH-terminal fragment of the heavy chain with an analogue of its ganglioside receptor, GT1b, provides the first direct identification and characterization of the ganglioside-binding sites. The ganglioside induces cross-linking by binding to two distinct sites on the Hc molecule. The structure sheds new light on the binding of Clostridial neurotoxins to receptors on neuronal cells and provides important information relevant to the design of anti-tetanus and anti-botulism therapeutic agents.

PubMed: 11418600
DOI: 10.1074/jbc.M103285200

実験手法

X-RAY DIFFRACTION (2.3 Å)