1FUU

YEAST INITIATION FACTOR 4A

1FUU の概要

• エントリーDOI: 10.2210/pdb1fuu/pdb
• 分子名称: YEAST INITIATION FACTOR 4A (2 entities in total)
• 機能のキーワード: if4a, helicase, dead-box protein, translation
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Cytoplasm: P10081
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 90261.27

構造登録者

Caruthers, J.M.,Johnson, E.R.,McKay, D.B. (登録日: 2000-09-15, 公開日: 2000-11-29, 最終更新日: 2024-11-20)

主引用文献

Caruthers, J.M.,Johnson, E.R.,McKay, D.B.
Crystal structure of yeast initiation factor 4A, a DEAD-box RNA helicase.
Proc.Natl.Acad.Sci.USA, 97:13080-13085, 2000

PubMed Abstract: The eukaryotic translation initiation factor 4A (eIF4A) is a member of the DEA(D/H)-box RNA helicase family, a diverse group of proteins that couples an ATPase activity to RNA binding and unwinding. Previous work has provided the structure of the amino-terminal, ATP-binding domain of eIF4A. Extending those results, we have solved the structure of the carboxyl-terminal domain of eIF4A with data to 1.75 A resolution; it has a parallel alpha-beta topology that superimposes, with minor variations, on the structures and conserved motifs of the equivalent domain in other, distantly related helicases. Using data to 2.8 A resolution and molecular replacement with the refined model of the carboxyl-terminal domain, we have completed the structure of full-length eIF4A; it is a "dumbbell" structure consisting of two compact domains connected by an extended linker. By using the structures of other helicases as a template, compact structures can be modeled for eIF4A that suggest (i) helicase motif IV binds RNA; (ii) Arg-298, which is conserved in the DEA(D/H)-box RNA helicase family but is absent from many other helicases, also binds RNA; and (iii) motifs V and VI "link" the carboxyl-terminal domain to the amino-terminal domain through interactions with ATP and the DEA(D/H) motif, providing a mechanism for coupling ATP binding and hydrolysis with conformational changes that modulate RNA binding.

PubMed: 11087862
DOI: 10.1073/pnas.97.24.13080

実験手法

X-RAY DIFFRACTION (2.5 Å)