1FT9

STRUCTURE OF THE REDUCED (FEII) CO-SENSING PROTEIN FROM R. RUBRUM

Summary for 1FT9

• Entry DOI: 10.2210/pdb1ft9/pdb
• Descriptor: CARBON MONOXIDE OXIDATION SYSTEM TRANSCRIPTION REGULATOR, PROTOPORPHYRIN IX CONTAINING FE (3 entities in total)
• Functional Keywords: carbon monoxide, heme sensor, catabolite gene activator protein, transcription
• Biological source: Rhodospirillum rubrum
• Total number of polymer chains: 2
• Total formula weight: 50560.87

Authors

Lanzilotta, W.N.,Schuller, D.J.,Poulos, T.L.,Thorsteinsson, M.V.,Kirby, B.,Roberts, G.P. (deposition date: 2000-09-12, release date: 2000-10-09, Last modification date: 2024-02-07)

Primary citation

Lanzilotta, W.N.,Schuller, D.J.,Thorsteinsson, M.V.,Kerby, R.L.,Roberts, G.P.,Poulos, T.L.
Structure of the CO sensing transcription activator CooA.
Nat.Struct.Biol., 7:876-880, 2000

PubMed Abstract: CooA is a homodimeric transcription factor that belongs to the catabolite activator protein (CAP) family. Binding of CO to the heme groups of CooA leads to the transcription of genes involved in CO oxidation in Rhodospirillum rubrum. The 2.6 A structure of reduced (Fe2+) CooA reveals that His 77 in both subunits provides one heme ligand while the N-terminal nitrogen of Pro 2 from the opposite subunit provides the other ligand. A structural comparison of CooA in the absence of effector and DNA (off state) with that of CAP in the effector and DNA bound state (on state) leads to a plausible model for the mechanism of allosteric control in this class of proteins as well as the CO dependent activation of CooA.

PubMed: 11017196
DOI: 10.1038/82820

Experimental method

X-RAY DIFFRACTION (2.6 Å)