1FPT

THREE-DIMENSIONAL STRUCTURE OF THE COMPLEX BETWEEN THE FAB FRAGMENT OF AN NEUTRALIZING ANTIBODY FOR TYPE 1 POLIOVIRUS AND ITS VIRAL EPITOPE

1FPT の概要

• エントリーDOI: 10.2210/pdb1fpt/pdb
• 分子名称: FAB FRAGMENT OF AN NEUTRALIZING ANTIBODY FOR TYPE 1 POLIOVIRUS, IGG2A-KAPPA C3 FAB (LIGHT CHAIN), IGG2A-KAPPA C3 FAB (HEAVY CHAIN), ... (4 entities in total)
• 機能のキーワード: complex (antibody-pv-1 fragment), complex (antibody-pv-1 fragment) complex, complex (antibody/pv-1 fragment)
• 由来する生物種: Human poliovirus 1; 詳細
• 細胞内の位置: Protein VP2: Virion. Protein VP3: Virion. Protein VP1: Virion. Protein 2B: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 2C: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 3A: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 3B: Virion (Potential). Picornain 3C: Host cytoplasm (Potential). RNA-directed RNA polymerase 3D-POL: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential): P03299; Cell membrane; Single-pass membrane protein (Potential): P01865
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 49499.09

構造登録者

Wien, M.W.,Hogle, J.M. (登録日: 1995-01-26, 公開日: 1995-03-31, 最終更新日: 2024-10-23)

主引用文献

Wien, M.W.,Filman, D.J.,Stura, E.A.,Guillot, S.,Delpeyroux, F.,Crainic, R.,Hogle, J.M.
Structure of the complex between the Fab fragment of a neutralizing antibody for type 1 poliovirus and its viral epitope.
Nat.Struct.Biol., 2:232-243, 1995

PubMed Abstract: The crystal structure of the complex between the Fab fragment of C3, a neutralizing antibody for poliovirus, and a peptide corresponding to the viral epitope has been determined at 3.0 A resolution. Although this antibody was originally raised to heat inactivated (noninfectious) virus particles, it strongly neutralizes the Mahoney strain of type 1 poliovirus. Eleven peptide residues are well-defined in the electron-density map and form two type I beta-turns in series. At the carboxyl end, the peptide is bound snugly in the antibody-combining site and adopts a conformation that differs significantly from the structure of the corresponding residues in the virus. Structural comparisons between the peptide in the complex and the viral epitope suggests that on binding to infectious virions, this antibody may induce structural changes important for neutralization.

PubMed: 7539711
DOI: 10.1038/nsb0395-232

実験手法

X-RAY DIFFRACTION (3 Å)