1FNH

CRYSTAL STRUCTURE OF HEPARIN AND INTEGRIN BINDING SEGMENT OF HUMAN FIBRONECTIN

1FNH の概要

• エントリーDOI: 10.2210/pdb1fnh/pdb
• 分子名称: PROTEIN (FIBRONECTIN) (2 entities in total)
• 機能のキーワード: heparin and integrin binding
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted, extracellular space, extracellular matrix: P02751
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29625.73

構造登録者

Sharma, A.,Askari, J.,Humphries, M.,Jones, E.Y.,Stuart, D.I. (登録日: 1999-01-28, 公開日: 1999-03-16, 最終更新日: 2023-12-27)

主引用文献

Sharma, A.,Askari, J.A.,Humphries, M.J.,Jones, E.Y.,Stuart, D.I.
Crystal structure of a heparin- and integrin-binding segment of human fibronectin.
EMBO J., 18:1468-1479, 1999

PubMed Abstract: The crystal structure of human fibronectin (FN) type III repeats 12-14 reveals the primary heparin-binding site, a clump of positively charged residues in FN13, and a putative minor site approximately 60 A away in FN14. The IDAPS motif implicated in integrin alpha4beta1 binding is at the FN13-14 junction, rendering the critical Asp184 inaccessible to integrin. Asp184 clamps the BC loop of FN14, whose sequence (PRARI) is reminiscent of the synergy sequence (PHSRN) of FN9. Mutagenesis studies prompted by this observation reveal that both arginines of the PRARI sequence are important for alpha4beta1 binding to FN12-14. The PRARI motif may represent a new class of integrin-binding sites. The spatial organization of the binding sites suggests that heparin and integrin may bind in concert.

PubMed: 10075919
DOI: 10.1093/emboj/18.6.1468

実験手法

X-RAY DIFFRACTION (2.8 Å)