1FML

CRYSTAL STRUCTURE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP

1FML の概要

• エントリーDOI: 10.2210/pdb1fml/pdb
• 関連するPDBエントリー: 1FMJ
• 分子名称: RETINOL DEHYDRATASE, CALCIUM ION, ADENOSINE-3'-5'-DIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: sulfotransferase, dehydratase, retinol, adenosine 3', 5'-diphosphate, transferase
• 由来する生物種: Spodoptera frugiperda (fall armyworm)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 84618.42

構造登録者

Pakhomova, S.,Kobayashi, M.,Buck, J.,Newcomer, M.E. (登録日: 2000-08-17, 公開日: 2001-05-02, 最終更新日: 2024-02-07)

主引用文献

Pakhomova, S.,Kobayashi, M.,Buck, J.,Newcomer, M.E.
A helical lid converts a sulfotransferase to a dehydratase.
Nat.Struct.Biol., 8:447-451, 2001

PubMed Abstract: We report here the crystal structure of retinol dehydratase, an enzyme that catalyzes the synthesis of anhydroretinol. The enzyme is a member of the sulfotransferase superfamily and its crystal structure reveals the insertion of a helical lid into a canonical sulfotransferase fold. Site-directed mutations demonstrate that this inserted lid is necessary for anhydroretinol production but not for sulfonation; thus, insertion of a helical lid can convert a sulfotransferase into a dehydratase.

PubMed: 11323722
DOI: 10.1038/87617

実験手法

X-RAY DIFFRACTION (2.75 Å)