1FLT

VEGF IN COMPLEX WITH DOMAIN 2 OF THE FLT-1 RECEPTOR

1FLT の概要

• エントリーDOI: 10.2210/pdb1flt/pdb
• 分子名称: VASCULAR ENDOTHELIAL GROWTH FACTOR, FMS-LIKE TYROSINE KINASE 1 (3 entities in total)
• 機能のキーワード: complex (growth factor-transferase), flt-1 receptor, cystine knot, glycoprotein, immunoglobulin-like domain transferase, complex (growth factor-transferase) complex, complex (growth factor/transferase)
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Secreted: P15692; Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Isoform 3: Secreted. Isoform 4: Secreted. Isoform 5: Cytoplasm (Potential). Isoform 6: Cytoplasm (Potential). Isoform 7: Cytoplasm (Potential): P17948
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 44835.82

構造登録者

Wiesmann, C.,De Vos, A.M. (登録日: 1997-11-20, 公開日: 1999-01-13, 最終更新日: 2024-10-30)

主引用文献

Wiesmann, C.,Fuh, G.,Christinger, H.W.,Eigenbrot, C.,Wells, J.A.,de Vos, A.M.
Crystal structure at 1.7 A resolution of VEGF in complex with domain 2 of the Flt-1 receptor.
Cell(Cambridge,Mass.), 91:695-704, 1997

PubMed Abstract: Vascular endothelial growth factor (VEGF) is a homodimeric hormone that induces proliferation of endothelial cells through binding to the kinase domain receptor and the Fms-like tyrosine kinase receptor (Flt-1), the extracellular portions of which consist of seven immunoglobulin domains. We show that the second and third domains of Flt-1 are necessary and sufficient for binding VEGF with near-native affinity, and that domain 2 alone binds only 60-fold less tightly than wild-type. The crystal structure of the complex between VEGF and the second domain of Flt-1 shows domain 2 in a predominantly hydrophobic interaction with the "poles" of the VEGF dimer. Based on this structure and on mutational data, we present a model of VEGF bound to the first four domains of Flt-1.

PubMed: 9393862
DOI: 10.1016/S0092-8674(00)80456-0

実験手法

X-RAY DIFFRACTION (1.7 Å)