1FLM

DIMER OF FMN-BINDING PROTEIN FROM DESULFOVIBRIO VULGARIS (MIYAZAKI F)

1FLM の概要

• エントリーDOI: 10.2210/pdb1flm/pdb
• 分子名称: PROTEIN (FMN-BINDING PROTEIN), FLAVIN MONONUCLEOTIDE (3 entities in total)
• 機能のキーワード: fmn binding, electron transport
• 由来する生物種: Desulfovibrio vulgaris str. 'Miyazaki F'
• 細胞内の位置: Cytoplasm: Q46604
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27218.82

構造登録者

Suto, K.,Kawagoe, K.,Shibata, N.,Morimoto, K.,Higuchi, Y.,Kitamura, M.,Nakaya, T.,Yasuoka, N. (登録日: 1999-03-10, 公開日: 2000-03-06, 最終更新日: 2023-12-27)

主引用文献

Suto, K.,Kawagoe, K.,Shibata, N.,Morimoto, Y.,Higuchi, Y.,Kitamura, M.,Nakaya, T.,Yasuoka, N.
How do the x-ray structure and the NMR structure of FMN-binding protein differ?
Acta Crystallogr.,Sect.D, 56:368-371, 2000

PubMed Abstract: The crystal structure of FMN-binding protein (FMN-bp) from Desulfovibrio vulgaris Miyazaki F was solved by the multiple isomorphous replacement method and refined to an R factor of 15.1% at 1.3 A resolution. FMN-bp exists in a dimeric form in the crystal, in contrast to the monomeric structure determined by NMR. R.m.s. deviations between the crystal structure and the solution structure are more than 2 A, which implies significant differences. There are some hydrophobic residues in the interface between the two monomers. In particular, Leu122 in the C-terminus has a close contact with the o-xylene moiety of FMN, while solvent molecules may cover the o-xylene moiety in the solution structure.

PubMed: 10713530
DOI: 10.1107/S0907444900000111

実験手法

X-RAY DIFFRACTION (1.3 Å)