1FLI

DNA-BINDING DOMAIN OF FLI-1

1FLI の概要

• エントリーDOI: 10.2210/pdb1fli/pdb
• 分子名称: FLI-1 (1 entity in total)
• 機能のキーワード: transcription/dna, transcription-dna complex
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: Q01543
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11444.93

構造登録者

Liang, H.,Mao, X.,Olejniczak, E.T.,Nettesheim, D.G.,Yu, L.,Meadows, R.P.,Thompson, C.B.,Fesik, S.W. (登録日: 1994-09-15, 公開日: 1995-09-15, 最終更新日: 2024-05-22)

主引用文献

Liang, H.,Mao, X.,Olejniczak, E.T.,Nettesheim, D.G.,Yu, L.,Meadows, R.P.,Thompson, C.B.,Fesik, S.W.
Solution structure of the ets domain of Fli-1 when bound to DNA.
Nat.Struct.Biol., 1:871-875, 1994

PubMed Abstract: Members of the ets family of transcription factors share a conserved DNA-binding domain, the ets domain. By using multidimensional NMR, we have determined the structure of the ets domain of human Fli-1 in the DNA-bound form. It consists of three alpha-helices and a four-stranded beta-sheet, similar to structures of the class of helix-turn-helix DNA binding proteins first found in the catabolite activator protein of Escherichia coli. NMR and mutagenesis experiments suggest that in comparison to structurally related proteins, the ets domain uses a new variation of the helix-turn-helix motif for binding to DNA.

PubMed: 7773776
DOI: 10.1038/nsb1294-871

実験手法

SOLUTION NMR