1FGB

TOXIN

1FGB の概要

• エントリーDOI: 10.2210/pdb1fgb/pdb
• 分子名称: CHOLERA TOXIN B SUBUNIT PENTAMER (2 entities in total)
• 機能のキーワード: cholera toxin, choleragenoid, enterotoxin, adp-ribosylation
• 由来する生物種: Vibrio cholerae
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 58116.33

構造登録者

Zhang, R.-G.,Westbrook, E. (登録日: 1996-02-21, 公開日: 1996-12-23, 最終更新日: 2024-10-30)

主引用文献

Zhang, R.G.,Westbrook, M.L.,Westbrook, E.M.,Scott, D.L.,Otwinowski, Z.,Maulik, P.R.,Reed, R.A.,Shipley, G.G.
The 2.4 A crystal structure of cholera toxin B subunit pentamer: choleragenoid.
J.Mol.Biol., 251:550-562, 1995

PubMed Abstract: Cholera toxin, a heterohexameric AB5 enterotoxin released by Vibrio cholera, induces a profuse secretory diarrhea in susceptible hosts. Choleragenoid, the B subunit pentamer of cholera toxin, directs the enzymatic A subunit to its target by binding the GM1 gangliosides exposed on the luminal surface of intestinal epithelial cells. The crystal structure of choleragenoid has been independently solved and refined at 2.4 A resolution by combining single isomorphous replacement with non-crystallographic symmetry averaging. The structure of the B subunits, and their pentameric arrangement, closely resembles that reported for the intact holotoxin, choleragen, the heat-labile enterotoxin from Escherichia coli, and for a choleragenoid-GM1 pentasaccharide complex. In the absence of the A subunit the central cavity of the B pentamer is a highly solvated channel. The binding of choleragenoid to the A subunit or to its receptor pentasaccharide modestly affects the local stereochemistry without perceptibly altering the subunit interface.

PubMed: 7658472
DOI: 10.1006/jmbi.1995.0455

実験手法

X-RAY DIFFRACTION (2.4 Å)