1FC6

PHOTOSYSTEM II D1 C-TERMINAL PROCESSING PROTEASE

1FC6 の概要

• エントリーDOI: 10.2210/pdb1fc6/pdb
• 関連するPDBエントリー: 1FC7 1FC9 1FCF
• 分子名称: PHOTOSYSTEM II D1 PROTEASE (2 entities in total)
• 機能のキーワード: d1 c-terminal processing protease, serine protease, serine-lysine catalytic dyad, pdz domain, photosystem ii, photosynthesis, x-ray crystal structure, hydrolase
• 由来する生物種: Scenedesmus obliquus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 41019.57

構造登録者

Liao, D.I.,Qian, J.,Chisholm, D.A.,Jordan, D.B.,Diner, B.A. (登録日: 2000-07-18, 公開日: 2001-01-18, 最終更新日: 2021-11-03)

主引用文献

Liao, D.I.,Qian, J.,Chisholm, D.A.,Jordan, D.B.,Diner, B.A.
Crystal structures of the photosystem II D1 C-terminal processing protease.
Nat.Struct.Biol., 7:749-753, 2000

PubMed Abstract: We report here the first three-dimensional structure of the D1 C-terminal processing protease (D1P), which is encoded by the ctpA gene. This enzyme removes the C-terminal extension of the D1 polypeptide of photosystem II of oxygenic photosynthesis. Proteolytic processing is necessary to allow the light driven assembly of the tetranuclear manganese cluster, which is responsible for photosynthetic water oxidation. The X-ray structure of the Scenedesmus obliquus enzyme has been determined at 1.8 A resolution using the multiwavelength anomalous dispersion method. The enzyme is monomeric and is composed of three folding domains. The middle domain is topologically homologous to known PDZ motifs and is proposed to be the site at which the substrate C-terminus binds. The remainder of the substrate likely extends across the face of the enzyme, interacting at its scissile bond with the enzyme active site Ser 372 / Lys 397 catalytic dyad, which lies at the center of the protein at the interface of the three domains.

PubMed: 10966643
DOI: 10.1038/78973

実験手法

X-RAY DIFFRACTION (1.8 Å)