1F99

CRYSTAL STRUCTURE OF R-PHYCOCYANIN FROM POLYSIPHONIA AT 2.4 A RESOLUTION

Summary for 1F99

• Entry DOI: 10.2210/pdb1f99/pdb
• Descriptor: R-PHYCOCYANIN, PHYCOCYANOBILIN, PHYCOERYTHROBILIN, ... (5 entities in total)
• Functional Keywords: light harvesting protein, r-phycocyanin, photosynthesis, electron transport
• Biological source: Polysiphonia urceolata; More
• Cellular location: Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Stromal side: P59858 P59859
• Total number of polymer chains: 6
• Total formula weight: 112098.87

Authors

Liang, D.C.,Jiang, T.,Chang, W.R. (deposition date: 2000-07-09, release date: 2001-07-09, Last modification date: 2025-03-12)

Primary citation

Jiang, T.,Zhang, J.P.,Chang, W.R.,Liang, D.C.
Crystal structure of R-phycocyanin and possible energy transfer pathways in the phycobilisome.
Biophys.J., 81:1171-1179, 2001

PubMed Abstract: The crystal structure of R-phycocyanin from Polysiphonia urceolata (R-PC-PU) at 2.4 A is reported. The R-PC-PU crystal belongs to space group P4(3)2(1)2 with cell parameters a = 135.1 A, c = 210.0 A, and alpha = beta = gamma = 90 degrees. The structure was determined by molecular replacement. The crystallographic R-factor of the refined model is 0.189 (R(free) = 0.239). Comparison of the microenvironment of chromophore beta 155 in R-PC-PU and in C-PC from Fremyolla diphosiphon (C-PC-FD) reveals that their spectral differences may be caused by their different alpha 28 residues. In the R-PC-PU crystal structure, two (alpha beta)(3) trimers assemble face to face to form a hexamer, and two such hexamers assemble in two novel side-to-side arrangements. Possible models for the energy transfer from phycoerythrin to phycocyanin and from phycocyanin to allophycocyanin are proposed based on several phycobiliprotein crystal structures.

PubMed: 11463658

Experimental method

X-RAY DIFFRACTION (2.4 Å)