1F8Y

CRYSTAL STRUCTURE ANALYSIS OF NUCLEOSIDE 2-DEOXYRIBOSYLTRANSFERASE COMPLEXED WITH 5-METHYL-2'-DEOXYPSEUDOURIDINE

Summary for 1F8Y

• Entry DOI: 10.2210/pdb1f8y/pdb
• Related: 1F8X
• Descriptor: NUCLEOSIDE 2-DEOXYRIBOSYLTRANSFERASE, 2'-deoxy-1-methyl-pseudouridine (3 entities in total)
• Functional Keywords: active site, alpha/beta protein, biocatalyst, nucleoside, transferase
• Biological source: Lactobacillus leichmannii
• Total number of polymer chains: 2
• Total formula weight: 36681.17

Authors

Armstrong, S.R.,Cook, W.J.,Short, S.A.,Ealick, S.E. (deposition date: 2000-07-05, release date: 2000-08-09, Last modification date: 2024-02-07)

Primary citation

Armstrong, S.R.,Cook, W.J.,Short, S.A.,Ealick, S.E.
Crystal structures of nucleoside 2-deoxyribosyltransferase in native and ligand-bound forms reveal architecture of the active site.
Structure, 4:97-107, 1996

PubMed Abstract: Nucleoside 2-deoxyribosyltransferase plays an important role in the salvage pathway of nucleotide metabolism in certain organisms, catalyzing the cleavage of beta-2'-deoxyribonucleosides and the subsequent transfer of the deoxyribosyl moiety to an acceptor purine or pyrimidine base. The kinetics describe a ping-pong-bi-bi pathway involving the formation of a covalent enzyme-deoxyribose intermediate. The enzyme is produced by a limited number of microorganisms and its functions have been exploited in its use as a biocatalyst to synthesize nucleoside analogs of therapeutic interest.

PubMed: 8805514
DOI: 10.1016/S0969-2126(96)00013-5

Experimental method

X-RAY DIFFRACTION (2.4 Å)