1F7Y

THE CRYSTAL STRUCTURE OF TWO UUCG LOOPS HIGHLIGHTS THE ROLE PLAYED BY 2'-HYDROXYL GROUPS IN ITS UNUSUAL STABILITY

1F7Y の概要

• エントリーDOI: 10.2210/pdb1f7y/pdb
• 分子名称: 16S RIBOSOMAL RNA FRAGMENT, 30S RIBOSOMAL PROTEIN S15, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: uucg, tetraloop, rna, ribosome
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29612.93

構造登録者

Ennifar, E.,Nikouline, A.,Serganov, A.,Tishchenko, S.,Nevskaya, N.,Garber, M.,Ehresmann, B.,Ehresmann, C.,Nikonov, S.,Dumas, P. (登録日: 2000-06-28, 公開日: 2000-11-22, 最終更新日: 2024-10-30)

主引用文献

Ennifar, E.,Nikulin, A.,Tishchenko, S.,Serganov, A.,Nevskaya, N.,Garber, M.,Ehresmann, B.,Ehresmann, C.,Nikonov, S.,Dumas, P.
The crystal structure of UUCG tetraloop.
J.Mol.Biol., 304:35-42, 2000

PubMed Abstract: All large structured RNAs contain hairpin motifs made of a stem closed by several looped nucleotides. The most frequent loop motif is the UUCG one. This motif belongs to the tetraloop family and has the peculiarity of being highly thermodynamically stable. Here, we report the first crystal structure of two UUCG tetraloops embedded in a larger RNA-protein complex solved at 2.8 A resolution. The two loops present in the asymmetric unit are in a different crystal packing environment but, nevertheless, have an identical conformation. The observed structure is globally close to that obtained in solution by nuclear magnetic resonance. However, subtle differences point to a more detailed picture of the role played by 2'-hydroxyl groups in stabilising this tetraloop.

PubMed: 11071808
DOI: 10.1006/jmbi.2000.4204

実験手法

X-RAY DIFFRACTION (2.8 Å)