1F27

CRYSTAL STRUCTURE OF A BIOTIN-BINDING RNA PSEUDOKNOT

1F27 の概要

• エントリーDOI: 10.2210/pdb1f27/pdb
• 分子名称: RNA (5'-R(*AP*CP*CP*GP*UP*CP*AP*GP*AP*GP*GP*AP*CP*AP*CP*GP*GP*UP*U)-3'), RNA (5'-R(*AP*AP*AP*AP*AP*GP*UP*CP*CP*UP*C)-3'), MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: rna aptamer, pseudoknot, biotin-binding rna, rna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 9981.01

構造登録者

Nix, J.,Sussman, D.,Wilson, C. (登録日: 2000-05-23, 公開日: 2000-06-12, 最終更新日: 2024-02-07)

主引用文献

Nix, J.,Sussman, D.,Wilson, C.
The 1.3 A crystal structure of a biotin-binding pseudoknot and the basis for RNA molecular recognition.
J.Mol.Biol., 296:1235-1244, 2000

PubMed Abstract: A pseudoknot-containing aptamer isolated from a pool of random sequence molecules has been shown previously to represent an optimal RNA solution to the problem of binding biotin. The affinity of this RNA molecule is nonetheless orders of magnitude weaker than that of its highly evolved protein analogs, avidin and streptavidin. To understand the structural basis for biotin binding and to compare directly strategies for ligand recognition available to proteins and RNA molecules, we have determined the 1.3 A crystal structure of the aptamer complexed with its ligand. Biotin is bound at the interface between the pseudoknot's stacked helices in a pocket defined almost entirely by base-paired nucleotides. In comparison to the protein avidin, the aptamer packs more tightly around the biotin headgroup and makes fewer contacts with its fatty acid tail. Whereas biotin is deeply buried within the hydrophobic core in the avidin complex, the aptamer relies on a combination of hydrated magnesium ions and immobilized water molecules to surround its ligand. In addition to demonstrating fundamentally different approaches to molecular recognition by proteins and RNA, the structure provides general insight into the mechanisms by which RNA function is mediated by divalent metals.

PubMed: 10698630
DOI: 10.1006/jmbi.2000.3539

実験手法

X-RAY DIFFRACTION (1.3 Å)