1EYX

CRYSTAL STRUCTURE OF R-PHYCOERYTHRIN AT 2.2 ANGSTROMS

1EYX の概要

• エントリーDOI: 10.2210/pdb1eyx/pdb
• 分子名称: R-PHYCOERYTHRIN, SULFATE ION, PHYCOCYANOBILIN, ... (7 entities in total)
• 機能のキーワード: r-phycoerythrin, macroseeding, twin, protein structure, sequences, phycobiliprotein, red algae, photosynthesis
• 由来する生物種: Gracilaria chilensis; 詳細
• 細胞内の位置: Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Stromal side: Q7SIG0 Q7SIF9
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 80106.56

構造登録者

Contreras-Martel, C.,Legrand, P.,Piras, C.,Vernede, X.,Martinez-Oyanedel, J.,Bunster, M.,Fontecilla-Camps, J.C. (登録日: 2000-05-09, 公開日: 2000-11-22, 最終更新日: 2025-03-12)

主引用文献

Contreras-Martel, C.,Martinez-Oyanedel, J.,Bunster, M.,Legrand, P.,Piras, C.,Vernede, X.,Fontecilla-Camps, J.C.
Crystallization and 2.2 A resolution structure of R-phycoerythrin from Gracilaria chilensis: a case of perfect hemihedral twinning.
Acta Crystallogr.,Sect.D, 57:52-60, 2001

PubMed Abstract: R-phycoerythrin, a light-harvesting component from the red algae Gracilaria chilensis, was crystallized by vapour diffusion using ammonium sulfate as precipitant agent. Red crystals grew after one week at 293 K and diffracted to 2.70 A resolution. Three serial macroseeding assays were necessary to grow a second larger crystal to dimensions of 0.68 x 0.16 x 0.16 mm. This crystal diffracted to 2.24 A resolution using synchrotron radiation at beamline BM14 of the European Synchrotron Radiation Facility (ESRF) at Grenoble, France and was used for structure determination. Data were collected at 100 K to a completeness of 98.6%. The crystal was trigonal, space group R3, with unit-cell parameters a = b = 187.3, c = 59.1 A, alpha = beta = 90, gamma = 120 degrees. Data treatment using the CCP4 suite of programs indicated that the crystal was twinned ((I(2))/(I)(2) = 1.41). Molecular replacement was performed with AMoRe using the R-phycoerythrin from Polysiphonia urceolata [Chang et al. (1996), J. Mol. Biol. 249, 424-440] as a search model. In order to overcome the twinning problem, SHELX97 was used for the crystallographic refinement. The twin fraction was 0.48, indicating a nearly perfect hemihedrally twinned crystal. The final R(work) and R(free) factors are 0.16 and 0.25, respectively. All the residues and chromophores of the alpha- and beta-chains are well defined in the electron-density maps. Some residues belonging to the gamma-linker are also recognizable.

PubMed: 11134927
DOI: 10.1107/S0907444900015274

実験手法

X-RAY DIFFRACTION (2.25 Å)