1EYG

Crystal structure of chymotryptic fragment of E. coli ssb bound to two 35-mer single strand DNAS

Summary for 1EYG

• Entry DOI: 10.2210/pdb1eyg/pdb
• Related: 1KAW
• Descriptor: SINGLE STRANDED 28-MER OF D(C), SINGLE-STRAND DNA-BINDING PROTEIN (3 entities in total)
• Functional Keywords: protein-dna complex; ob fold; se-met; mad phasing; ssb; binding mode, replication-dna complex, replication/dna
• Biological source: Escherichia coli
• Total number of polymer chains: 6
• Total formula weight: 71779.17

Authors

Raghunathan, S.,Waksman, G. (deposition date: 2000-05-06, release date: 2000-08-01, Last modification date: 2024-02-07)

Primary citation

Raghunathan, S.,Kozlov, A.G.,Lohman, T.M.,Waksman, G.
Structure of the DNA binding domain of E. coli SSB bound to ssDNA.
Nat.Struct.Biol., 7:648-652, 2000

PubMed Abstract: The structure of the homotetrameric DNA binding domain of the single stranded DNA binding protein from Escherichia coli (Eco SSB) bound to two 35-mer single stranded DNAs was determined to a resolution of 2.8 A. This structure describes the vast network of interactions that results in the extensive wrapping of single stranded DNA around the SSB tetramer and suggests a structural basis for its various binding modes.

PubMed: 10932248
DOI: 10.1038/77943

Experimental method

X-RAY DIFFRACTION (2.8 Å)