1EXB

STRUCTURE OF THE CYTOPLASMIC BETA SUBUNIT-T1 ASSEMBLY OF VOLTAGE-DEPENDENT K CHANNELS

1EXB の概要

• エントリーDOI: 10.2210/pdb1exb/pdb
• 関連するPDBエントリー: 1qrq 1t1d
• 分子名称: KV BETA2 PROTEIN, POTASSIUM CHANNEL KV1.1, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (4 entities in total)
• 機能のキーワード: ion channel, oxidoreductase, beta subunit, metal transport
• 由来する生物種: Rattus norvegicus (Norway rat); 詳細
• 細胞内の位置: Cytoplasm (Potential): P62483; Membrane; Multi-pass membrane protein: P10499
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 50314.26

構造登録者

Gulbis, J.M.,Zhou, M.,Mann, S.,MacKinnon, R. (登録日: 2000-05-02, 公開日: 2000-07-19, 最終更新日: 2024-02-07)

主引用文献

Gulbis, J.M.,Zhou, M.,Mann, S.,MacKinnon, R.
Structure of the cytoplasmic beta subunit-T1 assembly of voltage-dependent K+ channels.
Science, 289:123-127, 2000

PubMed Abstract: The structure of the cytoplasmic assembly of voltage-dependent K+ channels was solved by x-ray crystallography at 2.1 angstrom resolution. The assembly includes the cytoplasmic (T1) domain of the integral membrane alpha subunit together with the oxidoreductase beta subunit in a fourfold symmetric T1(4)beta4 complex. An electrophysiological assay showed that this complex is oriented with four T1 domains facing the transmembrane pore and four beta subunits facing the cytoplasm. The transmembrane pore communicates with the cytoplasm through lateral, negatively charged openings above the T1(4)beta4 complex. The inactivation peptides of voltage-dependent K(+) channels reach their site of action by entering these openings.

PubMed: 10884227
DOI: 10.1126/science.289.5476.123

実験手法

X-RAY DIFFRACTION (2.1 Å)