1EWY

ANABAENA PCC7119 FERREDOXIN:FERREDOXIN-NADP+-REDUCTASE COMPLEX

Summary for 1EWY

• Entry DOI: 10.2210/pdb1ewy/pdb
• Related: 1QT9 1QUE
• Descriptor: FERREDOXIN-NADP REDUCTASE, FERREDOXIN I, FLAVIN-ADENINE DINUCLEOTIDE, ... (5 entities in total)
• Functional Keywords: electron transfer complex, photosynthesis, protein-protein interaction, ferredoxin, reductase, oxidoreductase
• Biological source: Nostoc sp. PCC 7119; More
• Cellular location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side: P21890
• Total number of polymer chains: 3
• Total formula weight: 80537.86

Authors

Morales, R.,Charon, M.H.,Frey, M. (deposition date: 2000-04-28, release date: 2001-02-07, Last modification date: 2023-08-09)

Primary citation

Morales, R.,Kachalova, G.,Vellieux, F.,Charon, M.H.,Frey, M.
Crystallographic studies of the interaction between the ferredoxin-NADP+ reductase and ferredoxin from the cyanobacterium Anabaena: looking for the elusive ferredoxin molecule.
Acta Crystallogr.,Sect.D, 56:1408-1412, 2000

PubMed Abstract: Ferredoxin-NADP(+) reductase (FNR) and its physiological electron donor ferredoxin (Fd) from the cyanobacterium Anabaena PCC7119 have been co-crystallized. The unit-cell parameters are a = b = 63.72, c = 158.02 A and the space group is P2(1)2(1)2(1). The crystal structure has been solved with 2.4 A resolution synchrotron data by molecular replacement, anomalous dispersion and R(min) search methods. For the computations, the crystal was treated as a merohedral twin. The asymmetric unit contains two FNR molecules and one ferredoxin molecule. The packing of the FNR molecules displays a nearly tetragonal symmetry (space group P4(3)2(1)2), whereas the ferredoxin arrangement is orthorhombic. This study provides the first crystallographic model of a dissociable complex between FNR and Fd.

PubMed: 11053838
DOI: 10.1107/S0907444900010052

Experimental method

X-RAY DIFFRACTION (2.38 Å)