1EVW

L116A MUTANT OF THE HOMING ENDONUCLEASE I-PPOI COMPLEXED TO HOMING SITE DNA.

Summary for 1EVW

• Entry DOI: 10.2210/pdb1evw/pdb
• Related: 1A73 1CYQ 1CZ0 1EVX
• Descriptor: DNA (5'-D(*TP*GP*GP*CP*TP*AP*CP*CP*TP*TP*AP*A)-3'), DNA (5'-D(P*GP*AP*GP*AP*GP*TP*CP*A)-3'), DNA (5'-D(*TP*GP*AP*CP*TP*CP*TP*CP*TP*TP*AP*A)-3'), ... (7 entities in total)
• Functional Keywords: dna binding b-sheets; c-terminal exchanged dimer interface; bent dna, hydrolase-dna complex, hydrolase/dna
• Biological source: Physarum polycephalum; More
• Total number of polymer chains: 12
• Total formula weight: 96051.25

Authors

Galburt, E.A.,Jurica, M.S.,Chevalier, B.S.,Erho, D.,Stoddard, B.L. (deposition date: 2000-04-20, release date: 2000-08-03, Last modification date: 2024-02-07)

• Primary citation: Galburt, E.A.,Chadsey, M.S.,Jurica, M.S.,Chevalier, B.S.,Erho, D.,Tang, W.,Monnat Jr., R.J.,Stoddard, B.L.; Conformational changes and cleavage by the homing endonuclease I-PpoI: a critical role for a leucine residue in the active site.; J.Mol.Biol., 300:877-887, 2000; PubMed: 10891275; DOI: 10.1006/jmbi.2000.3874

Experimental method

X-RAY DIFFRACTION (3.1 Å)