1ES8

Crystal structure of free BglII

1ES8 の概要

• エントリーDOI: 10.2210/pdb1es8/pdb
• 関連するPDBエントリー: 1D2I 1DFM
• 分子名称: RESTRICTION ENDONUCLEASE BGLII, ACETIC ACID (3 entities in total)
• 機能のキーワード: restriction endonuclease, restriction enzyme, uncomplexed, hydrolase
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26091.81

構造登録者

Lukacs, C.M.,Aggarwal, A.K. (登録日: 2000-04-07, 公開日: 2001-01-17, 最終更新日: 2024-10-30)

主引用文献

Lukacs, C.M.,Kucera, R.,Schildkraut, I.,Aggarwal, A.K.
Structure of free BglII reveals an unprecedented scissor-like motion for opening an endonuclease.
Nat.Struct.Biol., 8:126-130, 2001

PubMed Abstract: Restriction endonuclease BglII completely encircles its target DNA, making contacts to both the major and minor grooves. To allow the DNA to enter and leave the binding cleft, the enzyme dimer has to rearrange. To understand how this occurs, we have solved the structure of the free enzyme at 2.3 A resolution, as a complement to our earlier work on the BglII-DNA complex. Unexpectedly, the enzyme opens by a dramatic 'scissor-like' motion, accompanied by a complete rearrangement of the alpha-helices at the dimer interface. Moreover, within each monomer, a set of residues--a 'lever'--lowers or raises to alternately sequester or expose the active site residues. Such an extreme difference in free versus complexed structures has not been reported for other restriction endonucleases. This elegant mechanism for capturing DNA may extend to other enzymes that encircle DNA.

PubMed: 11175900
DOI: 10.1038/84111

実験手法

X-RAY DIFFRACTION (2.3 Å)