1EP2

CRYSTAL STRUCTURE OF LACTOCOCCUS LACTIS DIHYDROOROTATE DEHYDROGENASE B COMPLEXED WITH OROTATE

1EP2 の概要

• エントリーDOI: 10.2210/pdb1ep2/pdb
• 関連するPDBエントリー: 1EP1 1EP3
• 分子名称: DIHYDROOROTATE DEHYDROGENASE B (PYRD SUBUNIT), DIHYDROOROTATE DEHYDROGENASE B (PYRK SUBUNIT), FLAVIN MONONUCLEOTIDE, ... (7 entities in total)
• 機能のキーワード: heterotetramer, oxidoreductase
• 由来する生物種: Lactococcus lactis; 詳細
• 細胞内の位置: Cytoplasm: P54322 P56968
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 63110.20

構造登録者

Rowland, P.,Norager, S.,Jensen, K.F.,Larsen, S. (登録日: 2000-03-27, 公開日: 2001-01-17, 最終更新日: 2024-02-07)

主引用文献

Rowland, P.,Norager, S.,Jensen, K.F.,Larsen, S.
Structure of dihydroorotate dehydrogenase B: electron transfer between two flavin groups bridged by an iron-sulphur cluster.
Structure Fold.Des., 8:1227-1238, 2000

PubMed Abstract: The fourth step and only redox reaction in pyrimidine de novo biosynthesis is catalyzed by the flavoprotein dihydroorotate dehydrogenase (DHOD). Based on their sequences, DHODs are grouped into two major families. Lactococcus lactis is one of the few organisms with two DHODs, A and B, belonging to each of the two subgroups of family 1. The B enzyme (DHODB) is a prototype for DHODs in Gram-positive bacteria that use NAD+ as the second substrate. DHODB is a heterotetramer composed of two different proteins (PyrDB and PyrK) and three different cofactors: FMN, FAD, and a [2Fe-2S] cluster.

PubMed: 11188687
DOI: 10.1016/S0969-2126(00)00530-X

実験手法

X-RAY DIFFRACTION (2.4 Å)