1EOO

ECORV BOUND TO COGNATE DNA

1EOO の概要

• エントリーDOI: 10.2210/pdb1eoo/pdb
• 分子名称: DNA (5'-D(*GP*AP*AP*GP*AP*TP*AP*TP*CP*TP*TP*C)-3'), TYPE II RESTRICTION ENZYME ECORV (3 entities in total)
• 機能のキーワード: protein-dna recognition, induced fit, endonuclease, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 64703.54

構造登録者

Horton, N.C.,Perona, J.J. (登録日: 2000-03-23, 公開日: 2000-04-04, 最終更新日: 2024-02-07)

主引用文献

Horton, N.C.,Perona, J.J.
Crystallographic snapshots along a protein-induced DNA-bending pathway.
Proc.Natl.Acad.Sci.USA, 97:5729-5734, 2000

PubMed Abstract: Two new high-resolution cocrystal structures of EcoRV endonuclease bound to DNA show that a large variation in DNA-bending angles is sampled in the ground state binary complex. Together with previous structures, these data reveal a contiguous series of protein conformational states delineating a specific trajectory for the induced-fit pathway. Rotation of the DNA-binding domains, together with movements of two symmetry-related helices binding in the minor groove, causes base unstacking at a key base-pair step and propagates structural changes that assemble the active sites. These structures suggest a complex mechanism for DNA bending that depends on forces generated by interacting protein segments, and on selective neutralization of phosphate charges along the inner face of the bent double helix.

PubMed: 10801972
DOI: 10.1073/pnas.090370797

実験手法

X-RAY DIFFRACTION (2.16 Å)