1EO6

CRYSTAL STRUCTURE OF GATE-16

1EO6 の概要

• エントリーDOI: 10.2210/pdb1eo6/pdb
• 分子名称: GOLGI-ASSOCIATED ATPASE ENHANCER OF 16 KD (2 entities in total)
• 機能のキーワード: ubiquitin fold, protein binding
• 由来する生物種: Bos taurus (cattle)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27373.67

構造登録者

Paz, Y.,Elazar, Z.,Fass, D. (登録日: 2000-03-22, 公開日: 2000-09-08, 最終更新日: 2024-02-07)

主引用文献

Paz, Y.,Elazar, Z.,Fass, D.
Structure of GATE-16, membrane transport modulator and mammalian ortholog of autophagocytosis factor Aut7p.
J.Biol.Chem., 275:25445-25450, 2000

PubMed Abstract: The GATE-16 protein participates in intra-Golgi transport and can associate with the N-ethylmaleimide-sensitive fusion protein and with Golgi SNAREs. The yeast ortholog of GATE-16 is the autophagocytosis factor Aut7p. GATE-16 is also closely related to the GABA receptor-associated protein (GABARAP), which has been proposed to cluster neurotransmitter receptors by mediating interaction with the cytoskeleton, and to the light chain-3 subunit of the neuronal microtubule-associated protein complex. Here, we present the crystal structure of GATE-16 refined to 1.8 A resolution. GATE-16 contains a ubiquitin fold decorated by two additional N-terminal helices. Proteins with strong structural similarity but no detectable sequence homology to GATE-16 include Ras effectors that mediate diverse downstream functions, but each interacts with Ras by forming pseudo-continuous beta-sheets. The GATE-16 surface suggests that it binds its targets in a similar manner. Moreover, a second potential protein-protein interaction site on GATE-16 may explain the adapter activity observed for members of the GATE-16 family.

PubMed: 10856287
DOI: 10.1074/jbc.C000307200

実験手法

X-RAY DIFFRACTION (1.8 Å)