1EJ4

COCRYSTAL STRUCTURE OF EIF4E/4E-BP1 PEPTIDE

1EJ4 の概要

• エントリーDOI: 10.2210/pdb1ej4/pdb
• 関連するPDBエントリー: 1EJ1 1EJH
• 分子名称: EUKARYOTIC INITIATION FACTOR 4E, EUKARYOTIC TRANSLATION INITIATION FACTOR 4E BINDING PROTEIN 1, 7N-METHYL-8-HYDROGUANOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: eif4e/4e-bp/7-methyl-gdp, translation
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 24464.59

構造登録者

Marcotrigiano, J.,Gingras, A.-C.,Sonenberg, N.,Burley, S.K. (登録日: 2000-02-28, 公開日: 2000-03-15, 最終更新日: 2024-03-06)

主引用文献

Marcotrigiano, J.,Gingras, A.C.,Sonenberg, N.,Burley, S.K.
Cap-dependent translation initiation in eukaryotes is regulated by a molecular mimic of eIF4G.
Mol.Cell, 3:707-716, 1999

PubMed Abstract: eIF4G uses a conserved Tyr-X-X-X-X-Leu-phi segment (where X is variable and phi is hydrophobic) to recognize eIF4E during cap-dependent translation initiation in eukaryotes. High-resolution X-ray crystallography and complementary biophysical methods have revealed that this eIF4E recognition motif undergoes a disorder-to-order transition, adopting an L-shaped, extended chain/alpha-helical conformation when it interacts with a phylogenetically invariant portion of the convex surface of eIF4E. Inhibitors of translation initiation known as eIF4E-binding proteins (4E-BPs) contain similar eIF4E recognition motifs. These molecules are molecular mimics of eIF4G, which act by occupying the same binding site on the convex dorsum of eIF4E and blocking assembly of the translation machinery. The implications of our results for translation initiation are discussed in detail, and a molecular mechanism for relief of translation inhibition following phosphorylation of the 4E-BPs is proposed.

PubMed: 10394359
DOI: 10.1016/S1097-2765(01)80003-4

実験手法

X-RAY DIFFRACTION (2.25 Å)