1EGK
CRYSTAL STRUCTURE OF A NUCLEIC ACID FOUR-WAY JUNCTION
Summary for 1EGK
| Entry DOI | 10.2210/pdb1egk/pdb |
| Related | 1BR3 |
| Descriptor | RNA (5'-R(*AP*GP*GP*AP*GP*AP*GP*AP*GP*AP*UP*GP*GP*GP*UP*GP*CP*GP*AP*G)-3'), 10-23 DNA ENZYME, MAGNESIUM ION, ... (4 entities in total) |
| Functional Keywords | four-way junction, nucleic acid, rna-dna complex, 10-23 dna enzyme, ribozyme, dna-rna complex, dna-rna hybrid |
| Total number of polymer chains | 4 |
| Total formula weight | 34151.58 |
| Authors | Nowakowski, J.,Shim, P.J.,Stout, C.D.,Joyce, G.F. (deposition date: 2000-02-15, release date: 2000-06-26, Last modification date: 2024-02-07) |
| Primary citation | Nowakowski, J.,Shim, P.J.,Stout, C.D.,Joyce, G.F. Alternative conformations of a nucleic acid four-way junction. J.Mol.Biol., 300:93-102, 2000 Cited by PubMed Abstract: A crystal structure of a 108 nucleotide RNA-DNA complex containing a four-way junction was solved at 3.1 A resolution. The structure of the junction differs substantially from the "stacked-X" conformation observed previously, due to a 135 degrees rotation of the branches. Comparison of the two conformers provides insight into the factors contributing to the flexibility of four-way junctions. The stacked-X conformation maximizes base-stacking but causes unfavorable repulsion between phosphate groups, whereas the 135 degrees -rotated "crossed" conformation minimizes electrostatic clashes at the expense of reduced base-stacking. Despite the large rotation of the branches, both junction structures exhibit an antiparallel arrangement of the continuous strands and opposite polarity of the crossover strands. PubMed: 10864501DOI: 10.1006/jmbi.2000.3826 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.1 Å) |
Structure validation
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