1EG7

THE CRYSTAL STRUCTURE OF FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICA

1EG7 の概要

• エントリーDOI: 10.2210/pdb1eg7/pdb
• 分子名称: FORMYLTETRAHYDROFOLATE SYNTHETASE, SULFATE ION (3 entities in total)
• 機能のキーワード: synthetase, folate binding, atp binding, formate binding, monovalent cation binding, ligase
• 由来する生物種: Moorella thermoacetica
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 120788.45

構造登録者

Radfar, R.,Shin, R.,Sheldrick, G.M.,Minor, W.,Lovell, C.R.,Odom, J.D.,Dunlap, R.B.,Lebioda, L. (登録日: 2000-02-14, 公開日: 2001-02-14, 最終更新日: 2024-02-07)

主引用文献

Radfar, R.,Shin, R.,Sheldrick, G.M.,Minor, W.,Lovell, C.R.,Odom, J.D.,Dunlap, R.B.,Lebioda, L.
The crystal structure of N(10)-formyltetrahydrofolate synthetase from Moorella thermoacetica.
Biochemistry, 39:3920-3926, 2000

PubMed Abstract: The structure was solved at 2.5 A resolution using multiwavelength anomalous dispersion (MAD) scattering by Se-Met residues. The subunit of N(10)-formyltetrahydrofolate synthetase is composed of three domains organized around three mixed beta-sheets. There are two cavities between adjacent domains. One of them was identified as the nucleotide binding site by homology modeling. The large domain contains a seven-stranded beta-sheet surrounded by helices on both sides. The second domain contains a five-stranded beta-sheet with two alpha-helices packed on one side while the other two are a wall of the active site cavity. The third domain contains a four-stranded beta-sheet forming a half-barrel. The concave side is covered by two helices while the convex side is another wall of the large cavity. Arg 97 is likely involved in formyl phosphate binding. The tetrameric molecule is relatively flat with the shape of the letter X, and the active sites are located at the end of the subunits far from the subunit interface.

PubMed: 10747779
DOI: 10.1021/bi992790z

実験手法

X-RAY DIFFRACTION (2.5 Å)