1EG0

FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME

1EG0 の概要

• エントリーDOI: 10.2210/pdb1eg0/pdb
• EMDBエントリー: 1003
• 分子名称: FRAGMENT OF 16S RRNA HELIX 23, PROTEIN (S15 RIBOSOMAL PROTEIN), PROTEIN (S17 RIBOSOMAL PROTEIN), ... (15 entities in total)
• 機能のキーワード: 70s ribosome, low resolution model, ribosome
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 224438.31

構造登録者

Gabashvili, I.S.,Agrawal, R.K.,Spahn, C.M.T.,Grassucci, R.A.,Svergun, D.I.,Frank, J.,Penczek, P. (登録日: 2000-02-11, 公開日: 2000-03-06, 最終更新日: 2024-02-07)

主引用文献

Gabashvili, I.S.,Agrawal, R.K.,Spahn, C.M.,Grassucci, R.A.,Svergun, D.I.,Frank, J.,Penczek, P.
Solution structure of the E. coli 70S ribosome at 11.5 A resolution.
Cell(Cambridge,Mass.), 100:537-549, 2000

PubMed Abstract: Over 73,000 projections of the E. coli ribosome bound with formyl-methionyl initiator tRNAf(Met) were used to obtain an 11.5 A cryo-electron microscopy map of the complex. This map allows identification of RNA helices, peripheral proteins, and intersubunit bridges. Comparison of double-stranded RNA regions and positions of proteins identified in both cryo-EM and X-ray maps indicates good overall agreement but points to rearrangements of ribosomal components required for the subunit association. Fitting of known components of the 50S stalk base region into the map defines the architecture of the GTPase-associated center and reveals a major change in the orientation of the alpha-sarcin-ricin loop. Analysis of the bridging connections between the subunits provides insight into the dynamic signaling mechanism between the ribosomal subunits.

PubMed: 10721991
DOI: 10.1016/S0092-8674(00)80690-X

実験手法

ELECTRON MICROSCOPY (11.5 Å)