1EDM

EPIDERMAL GROWTH FACTOR-LIKE DOMAIN FROM HUMAN FACTOR IX

1EDM の概要

• エントリーDOI: 10.2210/pdb1edm/pdb
• 分子名称: FACTOR IX, CALCIUM ION (3 entities in total)
• 機能のキーワード: epidermal growth factor, egf, calcium-binding, egf-like domain, structure and function, human factor ix, coagulation factor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P00740
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 8679.50

構造登録者

Rao, Z.,Handford, P.,Mayhew, M.,Knott, V.,Brownlee, G.G.,Stuart, D. (登録日: 1996-03-21, 公開日: 1996-10-14, 最終更新日: 2024-10-09)

主引用文献

Rao, Z.,Handford, P.,Mayhew, M.,Knott, V.,Brownlee, G.G.,Stuart, D.
The structure of a Ca(2+)-binding epidermal growth factor-like domain: its role in protein-protein interactions.
Cell(Cambridge,Mass.), 82:131-141, 1995

PubMed Abstract: Various diverse extracellular proteins possess Ca(2+)-binding epidermal growth factor (EGF)-like domains, the function of which remains uncertain. We have determined, at high resolution (1.5 A), the crystal structure of such a domain, from human clotting factor IX, as a complex with Ca2+. The Ca2+ ligands form a classic pentagonal bipyramid with six ligands contributed by one polypeptide chain and the seventh supplied by a neighboring EGF-like domain. The crystal structure identifies the role of Ca2+ in maintaining the conformation of the N-terminal region of the domain, but more importantly demonstrates that Ca2+ can directly mediate protein-protein contacts. The observed crystal packing of the domains provides a plausible model for the association of multiple tandemly linked EGF-like domains in proteins such as fibrillin-1, Notch, and protein S. This model is consistent with the known functional data and suggests a general biological role for these domains.

PubMed: 7606779
DOI: 10.1016/0092-8674(95)90059-4

実験手法

X-RAY DIFFRACTION (1.5 Å)