1EC4

SOLUTION STRUCTURE OF A HEXITOL NUCLEIC ACID DUPLEX WITH FOUR CONSECUTIVE T:T BASE PAIRS

1EC4 の概要

• エントリーDOI: 10.2210/pdb1ec4/pdb
• 関連するPDBエントリー: 205D 280D
• 分子名称: HEXITOL DODECANUCLEOTIDE (1 entity in total)
• 機能のキーワード: double helix, antisense, hexitol nucleic acid, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7627.38

構造登録者

Lescrinier, E.,Esnouf, R.M.,Schraml, J.,Busson, R.,Herdewijn, P. (登録日: 2000-01-25, 公開日: 2003-04-22, 最終更新日: 2024-05-22)

主引用文献

Lescrinier, E.,Esnouf, R.M.,Schraml, J.,Busson, R.,Heus, H.A.,Hilbers, C.W.,Herdewijn, P.
Solution structure of a HNA-RNA hybrid
Chem.Biol., 7:719-731, 2000

PubMed Abstract: Synthetic nucleic acid analogues with a conformationally restricted sugar-phosphate backbone are widely used in antisense strategies for biomedical and biochemical applications. The modified backbone protects the oligonucleotides against degradation within the living cell, which allows them to form stable duplexes with sequences in target mRNAs with the aim of arresting their translation. The biologically most active antisense oligonucleotides also trigger cleavage of the target RNA through activation of endogenous RNase H. Systematic studies of synthetic oligonucleotides have also been conducted to delineate the origin of the chirality of DNA and RNA that are both composed of D-nucleosides.

PubMed: 10980452
DOI: 10.1016/S1074-5521(00)00017-X

実験手法

SOLUTION NMR