1E9M

Ferredoxin VI from Rhodobacter Capsulatus

1E9M の概要

• エントリーDOI: 10.2210/pdb1e9m/pdb
• 分子名称: FERREDOXIN VI, FE2/S2 (INORGANIC) CLUSTER (3 entities in total)
• 機能のキーワード: iron-sulfur protein, ferredoxin, [2fe-2s], bacterium, electron transport
• 由来する生物種: RHODOBACTER CAPSULATUS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11590.85

構造登録者

Sainz, G.,Armengaud, J.,Stojanoff, V.,Sanishvili, N.,Jouanneau, Y.,Larry, S. (登録日: 2000-10-24, 公開日: 2001-04-09, 最終更新日: 2024-05-08)

主引用文献

Armengaud, J.,Sainz, G.,Jouanneau, Y.,Sieker, L.C.
Crystallization and Preliminary X-Ray Diffraction Analysis of a [2Fe-2S] Ferredoxin (Fdvi) from Rhodobacter Capsulatus
Acta Crystallogr.,Sect.D, 57:301-, 2001

PubMed Abstract: A [2Fe-2S] ferredoxin found in the photosynthetic bacterium Rhodobacter capsulatus has been purified in recombinant form from Escherichia coli. This protein, called FdVI, resembles ferredoxins involved in iron-sulfur cluster biosynthesis in various prokaryotic and eukaryotic cells. Purified recombinant FdVI was recovered in high yields and appeared to be indistinguishable from the genuine R. capsulatus ferredoxin based on UV-visible absorption and EPR spectroscopy and mass spectrometry. FdVI has been crystallized in the oxidized state by a sitting-drop vapour-diffusion technique using sodium formate as precipitant. Seeding larger drops from a previous hanging-drop-grown small crystal resulted in the formation of long red-brown prismatic needles. Preliminary X-ray diffraction analysis indicated that FdVI crystals are orthorhombic and belong to the space group P2(1)2(1)2(1), with unit-cell parameters a = 45.87, b = 49.83, c = 54.29 A.

PubMed: 11173487
DOI: 10.1107/S0907444900017832

実験手法

X-RAY DIFFRACTION (2.07 Å)