1E0R

Beta-apical domain of thermosome

1E0R の概要

• エントリーDOI: 10.2210/pdb1e0r/pdb
• 関連するPDBエントリー: 1A6D 1A6E 1ASS 1ASX
• 分子名称: THERMOSOME (1 entity in total)
• 機能のキーワード: chaperonin, hsp60, thermosome, tcp1, groel, thermoplasma acidophilum
• 由来する生物種: THERMOPLASMA ACIDOPHILUM
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17763.39

構造登録者

Bosch, G.,Baumeister, W.,Essen, L.-O. (登録日: 2000-04-06, 公開日: 2000-08-19, 最終更新日: 2023-12-06)

主引用文献

Bosch, G.,Baumeister, W.,Essen, L.-O.
Crystal Structure of the Beta-Apical Domain from Thermosome Reveals Structural Plasticity in Protrusion Region
J.Mol.Biol., 301:19-, 2000

PubMed Abstract: The crystal structure of the beta-apical domain of the thermosome, an archaeal group II chaperonin from Thermoplasma acidophilum, has been determined at 2.8 A resolution. The structure shows an invariant globular core from which a 25 A long protrusion emanates, composed of an elongated alpha-helix (H10) and a long extended stretch consisting of residues GluB245-ThrB253. A comparison with previous apical domain structures reveals a large segmental displacement of the protruding part of helix H10 via the hinge GluB276-ValB278. The region comprising residues GluB245-ThrB253 adopts an extended beta-like conformation rather than the alpha-helix seen in the alpha-apical domain. Consequently, it appears that the protrusions of the apical domains from group II chaperonins might assume a variety of context-dependent conformations during an open, substrate-accepting state of the chaperonin. Sequence variations in the protrusion regions that are found in the eukaryotic TRiC/CCT subunits may provide different structural propensities and hence serve different roles in substrate recognition.

PubMed: 10926489
DOI: 10.1006/JMBI.2000.3955

実験手法

X-RAY DIFFRACTION (2.8 Å)