1DZO

Truncated PAK pilin from Pseudomonas aeruginosa

1DZO の概要

• エントリーDOI: 10.2210/pdb1dzo/pdb
• 関連するPDBエントリー: 1NIL 1NIM 1PAJ 1PAK
• 分子名称: TYPE IV PILIN (2 entities in total)
• 機能のキーワード: lectin, adhesin, cell adhesion
• 由来する生物種: PSEUDOMONAS AERUGINOSA PAK
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12696.23

構造登録者

Hazes, B.,Read, R.J. (登録日: 2000-03-06, 公開日: 2000-06-11, 最終更新日: 2024-11-20)

主引用文献

Hazes, B.,Sastry, P.A.,Hayakawa, K.,Read, R.J.,Irvin, R.T.
Crystal Structure of Pseudomonas Aeruginosa Pak Pilin Suggests a Main-Chain-Dominated Mode of Receptor Binding
J.Mol.Biol., 299:1005-, 2000

PubMed Abstract: Fibers of pilin monomers (pili) form the dominant adhesin of Pseudomonas aeruginosa, and they play an important role in infections by this opportunistic bacterial pathogen. Blocking adhesion is therefore a target for vaccine development. The receptor-binding site is located in a C-terminal disulphide-bonded loop of each pilin monomer, but functional binding sites are displayed only at the tip of the pilus. A factor complicating vaccination is that different bacterial strains produce distinct, and sometimes highly divergent, pilin variants. It is surprising that all strains still appear to bind a common receptor, asialo-GM1. Here, we present the 1.63 A crystal structure of pilin from P. aeruginosa strain PAK. The structure shows that the proposed receptor-binding site is formed by two beta-turns that create a surface dominated by main-chain atoms. Receptor specificity could therefore be maintained, whilst allowing side-chain variation, if the main-chain conformation is conserved. The location of the binding site relative to the proposed packing of the pilus fiber raises new issues and suggests that the current fiber model may have to be reconsidered. Finally, the structure of the C-terminal disulphide-bonded loop will provide the template for the structure-based design of a consensus sequence vaccine.

PubMed: 10843854
DOI: 10.1006/JMBI.2000.3801

実験手法

X-RAY DIFFRACTION (1.63 Å)