1DVK

CRYSTAL STRUCTURE OF THE FUNCTIONAL DOMAIN OF THE SPLICING FACTOR PRP18

1DVK の概要

• エントリーDOI: 10.2210/pdb1dvk/pdb
• 分子名称: PRP18 (2 entities in total)
• 機能のキーワード: pre-mrna splicing factor, prp18, rna binding protein
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 39251.60

構造登録者

Jiang, J.,Horowitz, D.S.,Xu, R.M. (登録日: 2000-01-21, 公開日: 2000-04-05, 最終更新日: 2024-02-07)

主引用文献

Jiang, J.,Horowitz, D.S.,Xu, R.M.
Crystal structure of the functional domain of the splicing factor Prp18.
Proc.Natl.Acad.Sci.USA, 97:3022-3027, 2000

PubMed Abstract: The splicing factor Prp18 is required for the second step of pre-mRNA splicing. We have isolated and determined the crystal structure of a large fragment of the Saccharomyces cerevisiae Prp18 that lacks the N-terminal 79 amino acids. This fragment, called Prp18Delta79, is fully active in yeast splicing in vitro and includes the sequences of Prp18 that have been evolutionarily conserved. The core structure of Prp18Delta79 is compact and globular, consisting of five alpha-helices that adopt a novel fold that we have designated the five-helix X-bundle. The structure suggests that one face of Prp18 interacts with the splicing factor Slu7, whereas the more evolutionarily conserved amino acids in Prp18 form the opposite face. The most highly conserved region of Prp18, a nearly invariant stretch of 19 aa, forms part of a loop between two alpha-helices and may interact with the U5 small nuclear ribonucleoprotein particles. The structure is consistent with a model in which Prp18 forms a bridge between Slu7 and the U5 small nuclear ribonucleoprotein particles.

PubMed: 10737784
DOI: 10.1073/pnas.97.7.3022

実験手法

X-RAY DIFFRACTION (2.15 Å)