1DUD

DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDE HYDROLASE (D-UTPASE) COMPLEXED WITH THE SUBSTRATE ANALOGUE DEOXYURIDINE 5'-DIPHOSPHATE (D-UDP)

1DUD の概要

• エントリーDOI: 10.2210/pdb1dud/pdb
• 分子名称: DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE, DEOXYURIDINE-5'-DIPHOSPHATE (3 entities in total)
• 機能のキーワード: hydrolase, nucleotide metabolism, d-utpase, substrate analogue, d-udp complex
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16690.78

構造登録者

Larsson, G.,Svensson, L.A.,Nyman, P.O. (登録日: 1996-04-30, 公開日: 1996-11-08, 最終更新日: 2024-02-07)

主引用文献

Larsson, G.,Svensson, L.A.,Nyman, P.O.
Crystal structure of the Escherichia coli dUTPase in complex with a substrate analogue (dUDP).
Nat.Struct.Biol., 3:532-538, 1996

PubMed Abstract: We have determined the structure of the homotrimeric dUTPase from Escherichia coli, completed with an inhibitor and substrate analogue, dUDP. Three molecules of dUDP are found symmetrically bound per trimer, each in a shallow cleft between adjacent subunits, interacting with evolutionary conserved residues. The interactions of the uracil ring and the deoxypentose with the protein are consistent with the high specificity of the enzyme with respect to these groups. The positions of the two phosphate groups and adjacent water molecules are discussed in relation to the mechanism and kinetics of catalysis. The role that dUTPase plays in DNA metabolism makes the enzyme a potential target for chemotherapeutic drugs: the results presented here will aid in the design and development of inhibitory compounds.

PubMed: 8646539
DOI: 10.1038/nsb0696-532

実験手法

X-RAY DIFFRACTION (2.3 Å)