1DPX

STRUCTURE OF HEN EGG-WHITE LYSOZYME

1DPX の概要

• エントリーDOI: 10.2210/pdb1dpx/pdb
• 関連するPDBエントリー: 1DPW
• 分子名称: LYSOZYME, CHLORIDE ION (3 entities in total)
• 機能のキーワード: protein-chloride complex, hydrolase
• 由来する生物種: Gallus gallus (chicken)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14402.07

構造登録者

Weiss, M.S.,Palm, G.J.,Hilgenfeld, R. (登録日: 1999-12-28, 公開日: 2000-01-03, 最終更新日: 2024-11-20)

主引用文献

Weiss, M.S.,Palm, G.J.,Hilgenfeld, R.
Crystallization, structure solution and refinement of hen egg-white lysozyme at pH 8.0 in the presence of MPD.
Acta Crystallogr.,Sect.D, 56:952-958, 2000

PubMed Abstract: Hen egg-white lysozyme has been crystallized at slightly alkaline pH using 2-methyl-2,4-pentanediol (MPD) as the precipitant. The crystals are nearly isomorphous to crystals grown at acidic pH using sodium chloride as the precipitant. However, the growth kinetics differ markedly between the two conditions. The major reason for this is a molecule of MPD that binds tightly in between two lysozyme molecules and favors the growth of the crystals along the crystallographic c direction over growth perpendicular to it.

PubMed: 10944331
DOI: 10.1107/S0907444900006685

実験手法

X-RAY DIFFRACTION (1.65 Å)