1DP4

DIMERIZED HORMONE BINDING DOMAIN OF THE ATRIAL NATRIURETIC PEPTIDE RECEPTOR

1DP4 の概要

• エントリーDOI: 10.2210/pdb1dp4/pdb
• 分子名称: ATRIAL NATRIURETIC PEPTIDE RECEPTOR A, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, SULFATE ION, ... (6 entities in total)
• 機能のキーワード: periplasmic binding protein fold, dimer, hormone/growth factor receptor, lyase complex, hormone-growth factor receptor, lyase
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P18910
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 99723.94

構造登録者

van den Akker, F.,Zhang, X.,Miyagi, M.,Huo, X.,Misono, K.S.,Yee, V.C. (登録日: 1999-12-23, 公開日: 2000-07-12, 最終更新日: 2024-10-16)

主引用文献

van den Akker, F.,Zhang, X.,Miyagi, M.,Huo, X.,Misono, K.S.,Yee, V.C.
Structure of the dimerized hormone-binding domain of a guanylyl-cyclase-coupled receptor.
Nature, 406:101-104, 2000

PubMed Abstract: The atrial natriuretic peptide (ANP) hormone is secreted by the heart in response to an increase in blood pressure. ANP exhibits several potent anti-hypertensive actions in the kidney, adrenal gland and vascular system. These actions are induced by hormone binding extracellularly to the ANP receptor, thereby activating its intracellular guanylyl cyclase domain for the production of cyclic GMP. Here we present the crystal structure of the glycosylated dimerized hormone-binding domain of the ANP receptor at 2.0-A resolution. The monomer comprises two interconnected subdomains, each encompassing a central beta-sheet flanked by alpha-helices, and exhibits the type I periplasmic binding protein fold. Dimerization is mediated by the juxtaposition of four parallel helices, arranged two by two, which brings the two protruding carboxy termini into close relative proximity. From affinity labelling and mutagenesis studies, the ANP-binding site maps to the side of the dimer crevice and extends to near the dimer interface. A conserved chloride-binding site is located in the membrane distal domain, and we found that hormone binding is chloride dependent. These studies suggest mechanisms for hormone activation and the allostery of the ANP receptor.

PubMed: 10894551
DOI: 10.1038/35017602

実験手法

X-RAY DIFFRACTION (2 Å)