1DP2

CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN RHODANESE AND LIPOATE

1DP2 の概要

• エントリーDOI: 10.2210/pdb1dp2/pdb
• 関連するPDBエントリー: 1BOH 1BOI 1ORB 1RHD 1RHS 2ORA
• 分子名称: RHODANESE, 5-[(3S)-1,2-dithiolan-3-yl]pentanoic acid (3 entities in total)
• 機能のキーワード: rhodanese, liopate, sulfurtransferase, transferase
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Mitochondrion matrix: P00586
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33189.68

構造登録者

Zanotti, G.,Cianci, M. (登録日: 1999-12-23, 公開日: 2000-12-13, 最終更新日: 2024-11-06)

主引用文献

Cianci, M.,Gliubich, F.,Zanotti, G.,Berni, R.
Specific interaction of lipoate at the active site of rhodanese.
Biochim.Biophys.Acta, 1481:103-108, 2000

PubMed Abstract: Dihydrolipoate is an acceptor of the rhodanese-bound sulfane sulfur atom, as shown by analysis of the elementary steps of the reaction catalyzed by rhodanese. The crystal structure of sulfur-substituted rhodanese complexed with the non-reactive oxidized form of lipoate has revealed that the compound is bound at the enzyme active site, with the dithiolane ring buried in the interior of the cavity and the carboxylic end pointing towards the solvent. One of the sulfur atoms of the ligand in the unproductive complex is relatively close to the sulfane sulfur bound to Cys-247, the sulfur that is transferred during the catalytic reaction. This mode of binding of lipoate is likely to mimic that of dihydrolipoate. The results presented here support the possible role of dihydrolipoate as sulfur-acceptor substrate of rhodanese in an enzymatic reaction that might serve to provide iron-sulfur proteins with inorganic sulfide.

PubMed: 11004580
DOI: 10.1016/S0167-4838(00)00114-X

実験手法

X-RAY DIFFRACTION (2.01 Å)