1DOV

CRYSTAL STRUCTURE OF THE ALPHA-CATENIN DIMERIZATION DOMAIN

1DOV の概要

• エントリーDOI: 10.2210/pdb1dov/pdb
• 分子名称: ALPHA-CATENIN (1 entity in total)
• 機能のキーワード: four-helix bundle, cell adhesion
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19875.95

構造登録者

Pokutta, S.,Weis, W.I. (登録日: 1999-12-21, 公開日: 2000-07-12, 最終更新日: 2024-02-07)

主引用文献

Pokutta, S.,Weis, W.I.
Structure of the dimerization and beta-catenin-binding region of alpha-catenin.
Mol.Cell, 5:533-543, 2000

PubMed Abstract: In adherens junctions, alpha-catenin links the cadherin-beta-catenin complex to the actin-based cytoskeleton. alpha-catenin is a homodimer in solution, but forms a 1:1 heterodimer with beta-catenin. The crystal structure of the alpha-catenin dimerization domain, residues 82-279, shows that alpha-catenin dimerizes through formation of a four-helix bundle in which two antiparallel helices are contributed by each protomer. A slightly larger fragment, comprising residues 57-264, binds to beta-catenin. A chimera consisting of the alpha-catenin-binding region of beta-catenin linked to the amino terminus of alpha-catenin 57-264 behaves as a monomer in solution, as expected, since beta-catenin binding disrupts the alpha-catenin dimer. The crystal structure of this chimera reveals the interaction between alpha- and beta-catenin, and provides a basis for understanding adherens junction assembly.

PubMed: 10882138
DOI: 10.1016/S1097-2765(00)80447-5

実験手法

X-RAY DIFFRACTION (3 Å)