1DOK

MONOCYTE CHEMOATTRACTANT PROTEIN 1, P-FORM

1DOK の概要

• エントリーDOI: 10.2210/pdb1dok/pdb
• 分子名称: MONOCYTE CHEMOATTRACTANT PROTEIN 1, SULFATE ION (3 entities in total)
• 機能のキーワード: chemoattractant, cytokine
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P13500
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 17852.60

構造登録者

Lubkowski, J.,Bujacz, G.,Boque, L.,Wlodawer, A.,Domaille, P.J.,Handel, T.M. (登録日: 1996-11-27, 公開日: 1997-03-12, 最終更新日: 2024-10-16)

主引用文献

Lubkowski, J.,Bujacz, G.,Boque, L.,Domaille, P.J.,Handel, T.M.,Wlodawer, A.
The structure of MCP-1 in two crystal forms provides a rare example of variable quaternary interactions.
Nat.Struct.Biol., 4:64-69, 1997

PubMed Abstract: The X-ray crystal structure of recombinant human monocyte chemoattractant protein (MCP-1) has been solved in two crystal forms. One crystal form (P), refined to 1.85 A resolution, contains a dimer in the asymmetric unit, while the other (I) contains a monomer and was refined at 2.4 A. Although both crystal forms grow together in the same droplet, the respective quaternary structures of the protein differ dramatically. In addition, both X-ray structures differ to a similar extent from the solution structure of MCP-1. Such extent of variability of quaternary structures is unprecedented. In the crystal structures, the well-ordered N termini of MCP-1 form 3(10)-helices. Comparison of the three MCP-1 structures revealed a direct correlation between the main-chain conformation of the first two cysteine residues and the quaternary arrangements. These data can be used to explain the structural basis for the assignment of residues responsible for biological activity.

PubMed: 8989326
DOI: 10.1038/nsb0197-64

実験手法

X-RAY DIFFRACTION (1.85 Å)