1DOA

Structure of the rho family gtp-binding protein cdc42 in complex with the multifunctional regulator rhogdi

1DOA の概要

• エントリーDOI: 10.2210/pdb1doa/pdb
• 分子名称: PROTEIN (GTP-BINDING PROTEIN), PROTEIN (GDP-DISSOCIATION INHIBITOR 1), MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: gtp-binding protein, cdc42, rhogdi, cell cycle
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential): P60953; Cytoplasm: P19803
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 46654.44

構造登録者

Hoffman, G.R.,Nassar, N.,Cerione, R.C. (登録日: 1999-12-20, 公開日: 2000-02-09, 最終更新日: 2025-03-26)

主引用文献

Hoffman, G.R.,Nassar, N.,Cerione, R.A.
Structure of the Rho family GTP-binding protein Cdc42 in complex with the multifunctional regulator RhoGDI.
Cell(Cambridge,Mass.), 100:345-356, 2000

PubMed Abstract: The RhoGDI proteins serve as key multifunctional regulators of Rho family GTP-binding proteins. The 2.6 A X-ray crystallographic structure of the Cdc42/RhoGDI complex reveals two important sites of interaction between GDI and Cdc42. First, the amino-terminal regulatory arm of the GDI binds to the switch I and II domains of Cdc42 leading to the inhibition of both GDP dissociation and GTP hydrolysis. Second, the geranylgeranyl moiety of Cdc42 inserts into a hydrophobic pocket within the immunoglobulin-like domain of the GDI molecule leading to membrane release. The structural data demonstrate how GDIs serve as negative regulators of small GTP-binding proteins and how the isoprenoid moiety is utilized in this critical regulatory interaction.

PubMed: 10676816
DOI: 10.1016/S0092-8674(00)80670-4

実験手法

X-RAY DIFFRACTION (2.6 Å)