1DN6

THE CRYSTAL STRUCTURE OF D(GGATGGGAG). AN ESSENTIAL PART OF THE BINDING SITE FOR TRANSCRIPTION FACTOR IIIA

1DN6 の概要

• エントリーDOI: 10.2210/pdb1dn6/pdb
• 分子名称: DNA (5'-D(*GP*GP*AP*TP*GP*GP*GP*AP*G)-3'), DNA (5'-D(*CP*TP*CP*CP*CP*AP*TP*CP*C)-3') (2 entities in total)
• 機能のキーワード: a-dna, double helix, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 5472.61

構造登録者

McCall, M.,Brown, T.,Hunter, W.N.,Kennard, O. (登録日: 1987-05-11, 公開日: 1988-01-16, 最終更新日: 2024-02-07)

主引用文献

McCall, M.,Brown, T.,Hunter, W.N.,Kennard, O.
The crystal structure of d(GGATGGGAG): an essential part of the binding site for transcription factor IIIA.
Nature, 322:661-664, 1986

PubMed Abstract: Most genes in higher organisms are activated by the binding of proteins called transcription factors. One such protein, transcription factor IIIA (TFIIIA) from the frog, activates the gene for 5S RNA by binding to the region of the gene between nucleotides 45 and 97. This binding site has been defined by a variety of biochemical studies, including base-deletion experiments and DNase I footprinting. The protein also binds to the gene product: in immature frogs it is stored as a complex with 5S RNA. From the observation that TFIIIA can bind to either double-helical DNA or RNA, and from their own measurements, Rhodes and Klug have proposed that the DNA-binding site for TFIIIA has an RNA-like structure. Here we present the crystal structure analysis of a part of the DNA-binding site (nucleotides 81-89 of the gene) which forms a particularly strong interaction with the protein, and show that it has a conformation similar to the A' form of double-helical RNA.

PubMed: 3748146
DOI: 10.1038/322661a0

実験手法

X-RAY DIFFRACTION (3 Å)