1DFB

STRUCTURE OF A HUMAN MONOCLONAL ANTIBODY FAB FRAGMENT AGAINST GP41 OF HUMAN IMMUNODEFICIENCY VIRUS TYPE I

1DFB の概要

• エントリーDOI: 10.2210/pdb1dfb/pdb
• 分子名称: IGG1-KAPPA 3D6 FAB (LIGHT CHAIN), IGG1-KAPPA 3D6 FAB (HEAVY CHAIN) (2 entities in total)
• 機能のキーワード: immunoglobulin
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47714.25

構造登録者

He, X.M.,Rueker, F.,Casale, E.,Carter, D.C. (登録日: 1992-03-27, 公開日: 1993-10-31, 最終更新日: 2024-10-16)

主引用文献

He, X.M.,Ruker, F.,Casale, E.,Carter, D.C.
Structure of a human monoclonal antibody Fab fragment against gp41 of human immunodeficiency virus type 1.
Proc.Natl.Acad.Sci.USA, 89:7154-7158, 1992

PubMed Abstract: The three-dimensional structure of a human monoclonal antibody (Fab), which binds specifically to a major epitope of the transmembrane protein gp41 of the human immunodeficiency virus type 1, has been determined by crystallographic methods to a resolution of 2.7 A. It has been previously determined that this antibody recognizes the epitope SGKLICTTAVPWNAS, belongs to the subclass IgG1 (kappa), and exhibits antibody-dependent cellular cytotoxicity. The quaternary structure of the Fab is in an extended conformation with an elbow bend angle between the constant and variable domains of 175 degrees. Structurally, four of the hypervariable loops can be classified according to previously recognized canonical structures. The third hypervariable loops of the heavy (H3) and light chain (L3) are structurally distinct. Hypervariable loop H3, residues 102H-109H, is unusually extended from the surface. The complementarity-determining region forms a hydrophobic binding pocket that is created primarily from hypervariable loops L3, H3, and H2.

PubMed: 1496010
DOI: 10.1073/pnas.89.15.7154

実験手法

X-RAY DIFFRACTION (2.7 Å)