1DDR

MOLECULE: DIHYDROFOLATE REDUCTASE (E.C.1.5.1.3) COMPLEXED WITH METHOTREXATE AND UREA

1DDR の概要

• エントリーDOI: 10.2210/pdb1ddr/pdb
• 分子名称: DIHYDROFOLATE REDUCTASE, CHLORIDE ION, METHOTREXATE, ... (6 entities in total)
• 機能のキーワード: oxido-reductase, oxidoreductase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37358.82

構造登録者

Yennawar, H.P.,Farber, G.K. (登録日: 1995-06-29, 公開日: 1995-10-15, 最終更新日: 2024-02-07)

主引用文献

Dunbar, J.,Yennawar, H.P.,Banerjee, S.,Luo, J.,Farber, G.K.
The effect of denaturants on protein structure.
Protein Sci., 6:1727-1733, 1997

PubMed Abstract: Virtually all studies of the protein-folding reaction add either heat, acid, or a chemical denaturant to an aqueous protein solution in order to perturb the protein structure. When chemical denaturants are used, very high concentrations are usually necessary to observe any change in protein structure. In a solution with such high denaturant concentrations, both the structure of the protein and the structure of the solvent around the protein can be altered. X-ray crystallography is the obvious experimental technique to probe both types of changes. In this paper, we report the crystal structures of dihydrofolate reductase with urea and of ribonuclease A with guanidinium chloride. These two classic denaturants have similar effects on the native structure of the protein. The most important change that occurs is a reduction in the overall thermal factor. These structures offer a molecular explanation for the reduction in mobility. Although the reduction is observed only with the native enzyme in the crystal, a similar decrease in mobility has also been observed in the unfolded state in solution (Makhatadze G, Privalov PL. 1992. Protein interactions with urea and guanidinium chloride: A calorimetric study.

PubMed: 9260285

実験手法

X-RAY DIFFRACTION (2.45 Å)