1DDJ

CRYSTAL STRUCTURE OF HUMAN PLASMINOGEN CATALYTIC DOMAIN

1DDJ の概要

• エントリーDOI: 10.2210/pdb1ddj/pdb
• 分子名称: PLASMINOGEN (2 entities in total)
• 機能のキーワード: plasminogen, catalytic domain, blood clotting
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P00747
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 108320.54

構造登録者

Wang, X.,Terzyan, S.,Tang, J.,Loy, J.,Lin, X.,Zhang, X. (登録日: 1999-11-10, 公開日: 2000-02-18, 最終更新日: 2024-10-30)

主引用文献

Wang, X.,Terzyan, S.,Tang, J.,Loy, J.A.,Lin, X.,Zhang, X.C.
Human plasminogen catalytic domain undergoes an unusual conformational change upon activation.
J.Mol.Biol., 295:903-914, 2000

PubMed Abstract: Activation of the serine protease plasmin from its zymogen, plasminogen, is the key step in fibrinolysis leading to blood clot dissolution. It also plays critical roles in cell migration, such as in tumor metastasis. Here, we report the crystal structure of an inactive S741A mutant of human plasminogen catalytic domain at 2.0 A resolution. This structure permits a direct comparison with that of the plasmin catalytic unit. Unique conformational differences are present between these two structures that are not seen in other zymogen-enzyme pairs of the trypsin family. The functional significance of these differences and the structural basis of plasminogen activation is discussed in the light of this new structure.

PubMed: 10656799
DOI: 10.1006/jmbi.1999.3397

実験手法

X-RAY DIFFRACTION (2 Å)