1DCC

2.2 ANGSTROM STRUCTURE OF OXYPEROXIDASE: A MODEL FOR THE ENZYME:PEROXIDE COMPLEX

1DCC の概要

• エントリーDOI: 10.2210/pdb1dcc/pdb
• 関連するPDBエントリー: 1CCP 3CCP
• 分子名称: CYTOCHROME C PEROXIDASE, OXYGEN MOLECULE, PROTOPORPHYRIN IX CONTAINING FE, ... (4 entities in total)
• 機能のキーワード: oxidoreductase(h2o2(a))
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Mitochondrion matrix: P00431
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34379.06

構造登録者

Miller, M.A.,Shaw, A.,Kraut, J. (登録日: 1994-06-01, 公開日: 1994-08-31, 最終更新日: 2024-02-07)

主引用文献

Miller, M.A.,Shaw, A.,Kraut, J.
2.2 A structure of oxy-peroxidase as a model for the transient enzyme: peroxide complex.
Nat.Struct.Biol., 1:524-531, 1994

PubMed Abstract: The Fe+3-OOH complex of peroxidases has a very short half life, and its structure cannot be determined by conventional methods. The Fe+2-O2 complex provides a useful structural model for this intermediate, as it differs by only one electron and one proton from the transient Fe+3-OOH complex. We therefore determined the crystal structure of the Fe+2-O2 complex formed by a yeast cytochrome c peroxidase mutant with Trp 191 replaced by Phe. The refined structure shows that dioxygen can form a hydrogen bond with the conserved distal His residue, but not with the conserved distal Arg residue. When the transient Fe+3-OOH complex is modelled in a similar orientation, the active site of peroxidase appears to be optimized for catalysing proton transfer between the vicinal oxygen atoms of the peroxy-anion.

PubMed: 7664080
DOI: 10.1038/nsb0894-524

実験手法

X-RAY DIFFRACTION (2.2 Å)